Analysis of 5' junctions of human LINE-1 and Alu retrotransposons suggests an alternative model for 5'-end attachment requiring microhomology-mediated end-joining
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چکیده
منابع مشابه
Microhomology-mediated end joining induces hypermutagenesis at breakpoint junctions
Microhomology (MH) flanking a DNA double-strand break (DSB) drives chromosomal rearrangements but its role in mutagenesis has not yet been analyzed. Here we determined the mutation frequency of a URA3 reporter gene placed at multiple locations distal to a DSB, which is flanked by different sizes (15-, 18-, or 203-bp) of direct repeat sequences for efficient repair in budding yeast. Induction of...
متن کاملSynthesis-dependent microhomology-mediated end joining accounts for multiple types of repair junctions
Ku or DNA ligase 4-independent alternative end joining (alt-EJ) repair of DNA double-strand breaks (DSBs) frequently correlates with increased junctional microhomology. However, alt-EJ also produces junctions without microhomology (apparent blunt joins), and the exact role of microhomology in both alt-EJ and classical non-homologous end joining (NHEJ) remains unclear. To better understand the d...
متن کامل53BP1 promotes microhomology-mediated end-joining in G1-phase cells
Alternative non-homologous end joining (alt-NHEJ) was originally identified as a backup repair mechanism in the absence of classical NHEJ (c-NHEJ) factors but recent studies have demonstrated that alt-NHEJ is active even when c-NHEJ as well as homologous recombination is available. The functions of 53BP1 in NHEJ processes are not well understood. Here, we report that 53BP1 promotes DNA double-s...
متن کاملA role for human homologous recombination factors in suppressing microhomology-mediated end joining
DNA double-strand breaks (DSBs) are toxic lesions, which if improperly repaired can result in cell death or genomic instability. DSB repair is usually facilitated by the classical non-homologous end joining (C-NHEJ), or homologous recombination (HR) pathways. However, a mutagenic alternative NHEJ pathway, microhomology-mediated end joining (MMEJ), can also be deployed. While MMEJ is suppressed ...
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رابطه ی میانِ هنر و شرایطِ اجتماعیِ زایش آن همواره در طولِ تاریخ دغدغه ی ذهنی و دل مشغولیِ اساسیِ منتقدان و نیز هنرمندان بوده است. از آنجا که هنر در قفس آهنیِ زندگیِ اجتماعی محبوس است، گسترش وابستگیِ آن با نهاد ها و اصولِ اجتماعی پیرامون، صرفِ نظر از هم سو بودن و یا غیرِ هم سو بودنِ آن نهاد ها، امری اجتناب ناپذیر به نظر می رسد. با این وجود پدیدار گشتنِ چنین مباحثِ حائز اهمییتی در میان منتقدین، با ظهورِ مکتب ما...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2005
ISSN: 1088-9051
DOI: 10.1101/gr.3421505